CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Comparative evaluation of different methods for the detection of methicillin resistant staphylococcus aureus (mrsa) and its antibiotic susceptibility pattern

Author: 
Ramprasad B. Pal, Rashmi Joshi and Upasana Pathak
Subject Area: 
Health Sciences
Abstract: 

Background& Objectives: Methicillin resistant Staphylococcus aureus (MRSA) is majorly responsible for a variety of infections in healthcare setting and in general community. These infections range from mild skin boils or pimples to severe life threatening infection of deep tissues, lungs etc. MRSA is of concern not only because of its resistance to methicillin but also because it is generally resistant to many other chemotherapeutic agents like cephalosporin, macrolides and aminoglycosides. MRSA must be detected and treated aggressively to prevent secondary infections. This study will help to determine the simple, most specific and sensitive method for the detection of MRSA, which could be carried out in routine microbiology laboratory. Methods: Strains of Staphylococcus aureus isolated from different clinical specimens were included in the study, which were screened for methicillin resistance. Screening with oxacillin and cefoxitin antibiotic discs, oxacillin MIC using commercial E test strip and detection of mecA gene product PBP2a using Latex agglutination test was carried out. Antibiotic susceptibility test for all MRSA strains was carried out by Kirby Bauer Disc diffusion method as per CLSI guidelines to determine their sensitivity pattern against an array of antibiotics. Multiplex PCR of confirmed MRSA strains was carried out to study resistance genes. Results: In the present study 70 (74.468 %) S.aureus isolates were found to be methicillin resistant by AST with oxacillin (1μg) as well as cefoxitin (30μg), latex agglutination test identified 89 (94.680 %) as MRSA, whereas by oxacillin MIC by E-test only 55 (58.510 %) isolates were found to be MRSA and 74 (78.723%) carried the mecA gene which was determined by the PCR. Interpretation and Conclusion: Results of AST with both Oxacillin (1μg) and cefoxitin (30μg) were somewhat similar to that of PCR results, which is considered as gold standard for detection of methicillin resistance genes. The other investigated methods like latex agglutination, oxacillin E-test may not be appropriate because of the relatively lower levels of concordance with PCR results.

PDF file: 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran