CERTIFICATE

IMPACT FACTOR 2021

Subject Area

  • Life Sciences / Biology
  • Architecture / Building Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology & Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical Systems
  • Physics
  • Social Sciences & Humanities

Why Us? >>

  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly

Collagen Fibre orientation and behaviour of odontogenic cysts; A picrosirius red staining and polarising microscopic study

Author: 
Yogish, P., Girish, H. C., Sanjay Murgod, Sandesh, M., Asha and Kavitha, M
Subject Area: 
Health Sciences
Abstract: 

Introduction: Epithelial–mesenchymal interactions are thought to play an important role in the pathogenesis of odontogenic cysts. Odontogenic Keratocyst (OKC) is a benign cystic neoplasm with a characteristic parakeratinized epithelial lining, which differs histologically and behaviourally from the Dentigerous cyst (DC) and Radicular cyst (RC). Biological behaviour of OKC is dependent on both epithelium and the underlying stroma. Collagen plays an important role in maintaining structural integrity and in determination of tissue functions. Collagen has natural birefringence which is attributed to arrangement of its fibres; this property is enhanced by Picrosirius Red staining. Aim: The purpose of this study will be to investigate the differences in collagen fibres within the fibrous tissue walls of OKC, DC and RC. Study design: Formalin-fixed paraffin-embedded tissue samples of OKC, DC, RC was segregated from the department of Oral Pathology & Microbiology, RRDCH and cut sections was subjected to staining with Picrosirius red and was observed under polarising light microscope. Polarising colours of the collagen fibres was recorded and statistically analysed. Materials and Methods: A group of 30 histopathologically diagnosed cases of Odontogenic cysts, 10 cases of Radicular cysts, 10 cases of Dentigerous cyst, 10 cases of Odontogenic Keratocyst and 2 cases of Dental follicular tissue as controls were retrieved from files of Department of Oral Pathology and Microbiology, Rajarajeswari Dental College and Hospital. Haematoxylin and Eosin stained slides were evaluated for the existence and degree of inflammation in all the slides. Paraffin embedded tissue blocks were sectioned at 5µm thickness. Sections were deparaffinised, hydrated and stained with Picro Sirius Red stain for collagen. Statistical analysis: Level of Significance: α=0.05 Statistical test used: Chi-squared (χ2) test Results: Our study showed that in, RC and DC - yellowish orange and orange color was seen indicating dense fibrosis in case of mild and moderate inflammation. Whereas in case of OKC , greenish yellow color was dominant in subepithelial, intermediate layer in absence and mild inflammation, whereas in periphery yellowish orange was seen in mild inflammation Conclusion: This method can be used to analyze the orientation of collagen fibers and also how inflammation can induce a change in the collagen from loosely packed thin fibrils to closely packed mature collagen. It can also be used as a diagnostic tool to differentiate two lesions and to predict their nature in terms of biologic behaviour and prognosis.

PDF file: 

CALL FOR PAPERS

 

ONLINE PAYPAL PAYMENT

IJMCE RECOMMENDATION

Advantages of IJCR

  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High Qualitiy reader base
  • High level author suport

Plagiarism Detection

IJCR is following an instant policy on rejection those received papers with plagiarism rate of more than 20%. So, All of authors and contributors must check their papers before submission to making assurance of following our anti-plagiarism policies.

 

EDITORIAL BOARD

CHUDE NKIRU PATRICIA
Nigeria
Dr. Swamy KRM
India
Dr. Abdul Hannan A.M.S
Saudi Arabia.
Luai Farhan Zghair
Iraq
Hasan Ali Abed Al-Zu’bi
Jordanian
Fredrick OJIJA
Tanzanian
Firuza M. Tursunkhodjaeva
Uzbekistan
Faraz Ahmed Farooqi
Saudi Arabia
Eric Randy Reyes Politud
Philippines
Elsadig Gasoom FadelAlla Elbashir
Sudan
Eapen, Asha Sarah
United State
Dr.Arun Kumar A
India
Dr. Zafar Iqbal
Pakistan
Dr. SHAHERA S.PATEL
India
Dr. Ruchika Khanna
India
Dr. Recep TAS
Turkey
Dr. Rasha Ali Eldeeb
Egypt
Dr. Pralhad Kanhaiyalal Rahangdale
India
DR. PATRICK D. CERNA
Philippines
Dr. Nicolas Padilla- Raygoza
Mexico
Dr. Mustafa Y. G. Younis
Libiya
Dr. Muhammad shoaib Ahmedani
Saudi Arabia
DR. MUHAMMAD ISMAIL MOHMAND
United State
DR. MAHESH SHIVAJI CHAVAN
India
DR. M. ARUNA
India
Dr. Lim Gee Nee
Malaysia
Dr. Jatinder Pal Singh Chawla
India
DR. IRAM BOKHARI
Pakistan
Dr. FARHAT NAZ RAHMAN
Pakistan
Dr. Devendra kumar Gupta
India
Dr. ASHWANI KUMAR DUBEY
India
Dr. Ali Seidi
Iran
Dr. Achmad Choerudin
Indonesia
Dr Ashok Kumar Verma
India
Thi Mong Diep NGUYEN
France
Dr. Muhammad Akram
Pakistan
Dr. Imran Azad
Oman
Dr. Meenakshi Malik
India
Aseel Hadi Hamzah
Iraq
Anam Bhatti
Malaysia
Md. Amir Hossain
Bangladesh
Ahmet İPEKÇİ
Turkey
Mirzadi Gohari
Iran