Antioxidant and invitro cytotoxicity of toddalia asiatica l

Whole plant of Toddalia asiatica were selected to evaluate antioxidant activities and invitro cytotoxic activity using commonly accepted assays. They were extracted with chloroform, methanol and petroleum ether respectively and selected for the best antioxidant and cytotoxic results. Flavonoids, such as quercetin, rutin, and antioxidant BHA (Butylated Hydroxyanisole) were included and used as standards in this study. For all the assays concentrations of samples are maintained from 62.5 to 1000 µg/ml. Each sample under assay condition showed a dose-dependent antioxidant effect of DPPH (1,1-diphenyl-2-picryl hydrazyl radical), FRAP (Ferric reducing ability of plasma) and LPO (Lipid peroxidation inhibitory activity). Among the crude plant extracts, Methanol extract showed stronger IC50 values in antioxidant studies in DPPH and LPO methods. IC50 of Methanolic extract in DPPH is 500 ±0.00 followed by Pet.ether and Chloroform, IC50 of Pet.ether extract in LPO is 300±0.00 followed by Chloroform and Methanol. Overall FRAP absorbance range is 0.051 – 0.472, all the extracts showed moderate reducing power. The extracts also showed dose dependent cytotoxic effect on normal (Vero) and cancer cell lines (HaCaT,A549,HeLa,HT-29) when analyzed by MTT assay. Of the different crude extracts, extracts demonstrated maximum cytotoxicity to cancer cell lines, CTC50 values ranging from 116.67± 5.8 to 710 ± 10, and all the extracts showed high toxicity toward normal cell line Vero. Nearly all cancer cells could be killed by extracts, where as small fractions of cells from cancer cell lines showed resistance even at much higher concentrations.