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The ability of local strains of pseudomonas fluorescens isolated from different agricultural area to produce four secondary metabolites

Author:

Asaad M. Ridha Al-Taee, Muhammad A. Fayadh and Mustafa S. Mahdi

Subject Area:

Life Sciences

Abstract:

In this study, 24 local isolates of Pseudomonas fluorescens have been isolatedfrom soil of different agricultural areas during the period from January to February 2011. The bacteria were identified using biochemical tests and the isolates were tested for their ability to produce secondary metabolites. The secondary metabolites have been identified using gas chromatography mass spectrophotometry (GC-MS), which revealed the four compounds as: (E) dodec-3-ene M.W. 168.32 Dalton, (R )-2 ((s)-amino-4-methylpentanamido)- 4-methypentanoic acid M.W. 24433 Dalton, (s)-hexahydropyrrolo (1,2-a)pyrazine-14-dione M.W. 154017 Dalton, and 3-isobutylhexahydropyrrolo(1,2-a)pyrazine -1,4-dione M. W. 210.27 Dalton. The first two compounds have been isolatedfor first time.

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