Bacteriocin typing of staphylococcus Aureus isolated from clinical specimen

The antibacterial activity of local isolates frome isolated from Baghdad, Iraq samples of different sources urine and wounds, ear and eye swab(25)strain of Staphylococcus aureus. From the collected clinical samples that gave positive result in coagulase of it was (MRSA methicillinresistance S. aureus 1 according to sensitivity test and vitek 2 system. Bacteriocin synthesis is a valuable character of some staphylococcal strains. Staphylococcal bacteriocinsa broad activity spectrum against many Gram-positive and Gram-negative bacteria are lethal to strains belonging to the same or related species as well as have. On the other hand, studies on the possibility of typing S. aureus MRSA, on the basis of their sensitivity to bacteriocins, are rarely published. Therefore, the purpose of this study was to use the production of bacteriocin from active strains in typing of S. aureus. A total of 10S. aureus were isolated from wound infections. Four staphylococci isolates (S. aureus1) were selected on the basis of sensitivity to most antibiotics which were used as basic indicator strains to determine the most producing staphylococcin isolates. (S. aureus 1) were chosen as good Staphylococcin producers according to their widest inhibition zone on the basic indicator isolates. Then the 10 isolates (producers) were tested against (Indicator) by well diffusion method. Staphylococcin of S. aureus 1, 5,7,8,11,16,18,22,23,25 strains inhibited of the tested isolates respectively (from wound infaction) multiple resistant strains that produced largest inhibition zone against the indicator strain was chosen for further study,