Sea mango leaf and fruit (Cerbera manghas, Linn) are very toxic, therefore some people in Southeast Asia and South Asia use them as fish poisons and rat poisons, besides that some people have also used them to treat several diseases, such as, diabetes, digestion, fever, malaria and infectious diseases. This study aims to determine the potential of dichloromethane and ethyl acetate extracts of sea mango (Cerbera manghas, linn) leaf against Staphylococcus aureus and Escherichia coli. The leaves of Cerbera manghas, Linn was obtained from Research Institute for Spices and Medicinal Plants (BALITRO) Bogor, Indonesia. Sea mango leaves were extracted by multistage extraction using dichloromethane and ethyl acetate solvents. The obtained extracts were prepared with concentrations of 10 mg/ml, 20 mg/ml, and 30 mg/ml. Test preparation activity were carried out against Escherichia coli and Staphylococcus aureus by using paper disc and dilution methods, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were determined and ampicillin antibiotic was used as a comparison. In this cases was obtained the MIC values of ethyl acetate extract against E coli and S aureus was 5 mg/ml and 2,5 mg/ml respectively and for dichloromethane extract was 2,5 mg/ml and 1,5 mg/ml respectively, while the MBC values of ethyl acetate extract against E coli and S aureus was 10 mg/ml and 5 mg/ml respectively and for dichloromethane extract was 5 mg/ml and 2,5 mg/ml respectively. Equality value for 10 - 30 mg/ml extract of ethyl acetate was same with 26,12 - 36,20 µg/ml ampicillin in against E. coli and 10,37 - 15,55µg/ml in against S. aureus respectively. Equality value for 10 - 30 mg/ml extract of dichloromethane was same with 20,55 - 34,98 µg/ml l ampicillin in against E. coli and 5,17 - 13,4 µg/ml in against S. aureus respectively. Based on the results of this study was obtained, that extracts of ethyl acetate and dichloromethane sea mango (Cerbera manghas, Linn) leaves have antibacterial activity against S aureus and E coli, where, extracts dichloromethane leaf of sea mango has antibacterial activity a little stronger than the extract ethyl acetate sea mango leaf.
