The effects of in vitro culture on the leaf anatomy of potato (solanum tuberosum l. cv. arizaona)

The leaves of Solanum tuberosum has been studied in plants cultivated both in vitro and ex vitro under illuminated growth chamber conditions during acclamation period, as well as conventionally plants in natural conditions. These conditions induce anatomical alterations, the results showed that number of stomata in the acclimatization plants greater than in the conventionally plants. The results showed that similarity plantlets producing from tuber in vitro with field plants in some anatomical characteristic. There are two types of stomatal types in plantlets producing from explant, while found five types in plantlets produced from tuber and field plants. As well as the results showed that palisade and spongy layers had greater air space within it and the cells of these leaves were irregular shape. In contrast, the anatomy of leaves under plantlets produced from tuber and conventionally plants were noticed reduce air space between cells and the cells more arrangement. In plantlets produced from explantmidrib contains continuous layer of palisade layer which losing in other treatments.