Factors influencing the ramie cellulase synthesis in aspergillus flavus by assessment of decortication and degumming

Ramie (Boehmeria nivea Gaud) a member of Utricacease, yields fibres which are longest and toughest. These fibres showed highest resistant to the action of water and are use industrial point of view in paper and textile industry. A total of 20 fungal strains were isolated from Ramie canes. Of the 20 there are six strains were identified as Aspergillus species. Among the Six strains Aspergillus flavus showed maximum colony count at 72 hours of incubation. In the present study deals with the isolation and screening of freshly isolated potent fungal strain as Aspergillus flavus for the production of cellulase enzyme consisting of the endoglucanase. Assessment of Decortication and degumming also recorded in cellulase enzyme production by Aspergillus flavus. Further the studies were undertaken on the optimization of physiological and nutritional culture conditions in respect of pH, incubation time, and temperature. Maximum cellulase production was recorded with 9 days of incubation time, optimum pH 7.0 and 350 C temperature. Synthesis of maximum endoglucanase 77.8% viscosity loss at 12 days of age. Aspergillus flavus showed the production of higher activity of cellulase enzyme consisting of endoglucanase yielded higher cellulase enzyme activity.