Apoptotic and antioxidative status of sperm in diabetic patients of 25-40 years age group under duration dependent regular and irregular mode of treatment: a comparative approach of proteomic and genomic study

Objective: The present study was undertaken to search out the status of antioxidant enzymes, apoptotic markers of sperm pellet and their molecular mechanism study in the diabetic patients under regular and irregular mode of different duration dependent treatment protocol within 25-40 years age group. Methods: Blood glycemic sensors, seminal catalase, superoxide dismutase (SOD), Bax, Bcl-2 protein, transcriptions of catalase, SOD, Bax and Bcl-2 genes in sperm were evaluated. Results: Levels of blood glycemic sensors, activities of antioxidative enzymes like catalase and superoxide dismutase and translation value of apoptatic markers like Bax protein, Bcl-2 protein, and transcriptions of catalse, SOD, Bax and Bcl-2 genes in sperm was corrected significantly after treatment of diabetic patients under regular mode for 4 to 5 years though not up to the control level. When the duration of the said treatment was extended beyond 4-5 years up to 15 years there are no significant recovery further in this line. In contrast, the levels of these sensors were shifted towards the pathological direction further along with the extension of the duration of the irregular treatment more in comparison to the irregular pattern treatment for 4-5 year of the diabetic patients.. Conclusion: So, it may conclude that regular mode of treatment is more effective for correction of spermiological sensors in diabetic patients in respect to irregular mode of treatment. Moreover, irregular mode of treatment with prolog duration may exert more adverse effect on spermiological sensors in diabetic patients.