Impact of syzigium lineare wall. (myrtaceae) leaf extracts on haemolymph protein profile of the armyworm, spodoptera litura (fab.) (lepidoptera: noctuidae)

Quantitative and qualitative changes of haemolymph protein profile of S. litura was studied under the stress of subfractions isolated from S. lineare. The study was conducted at Entomolgy Research Institute, Loyola College, Chennai, India. Standard protcol was followed to estimate qualitative and quantitatiive changes of haemolymph protein. The estimation was done after fourth instar larvae of S. litura fed on castor leaves treated with 25, 50, 75 and 100ppm concentration of promising sub fractions II, III, IV and VI for 24h. Protein content in haemolymph of S. litura was reduced significantly (P<0.05; by LSD) in all treatments. The reduction of protein in sub fraction II and positive control azadiracthin did not show any statistical significance (P>0.05; by LSD). Maximum qualitative changes were observed only on storage proteins with the molecular weight of 68KD. The concentration of storage protein was reduced maximum in sub fraction II and III at 25ppm concentration and it was increased in sub fraction III at 100ppm and sub fraction IV at 25ppm concentration respectively compared to control. The dissimilarity in qualitative and quantitative changes may be associated with differences in biological and biochemical activities in the larvae of S. litura to overcome the stress of S. lineare.