Study on effect of heavy metal lead on germinal cells & behavioural pattern of male mus musculus

Numerous heavy metals show significant mutagenic, teratogenic, and clastogenic. Most of the heavy metals such as lead, arsenic, mercury, and cadmium affect on male reproductive function, including sperm head morphology and spermatogenesis. Several studies have reported that heavy metals caused testicular oxidative stress, which has been linked to infertility. The aim of the present research his to investigate the cytotoxic effect of lead acetate on production of sperm cells and behavioural pattern during dosing period of two doses (20 and 40 mg/kg body weight/ per day) of lead acetate for 40 days in male Swiss albino mice (Mus musculus). For the research Adult Male Mus musculus of same age and average body weight 25 -30 gm taken and were divided into three groups one group was considered control group and other two were considered treated groups. Sperm counting and behaviour pattern parameter were included in this research to investigate the cytotoxic effect of lead acetate. After 40 days of treatment, the experimental animals were sacrificed and epididymal part were taken for study of sperm counting. The result shows that in both lower dose and higher dose of lead acetate were significantly decline the sperm counting (p < 0.05). The number of sperms was 150.4 ± 3.12 (control), 87.08 ± 2.21 (lower dose), and 48.1 ± 1.4 (higher dose) million cells/ml. In behavioural pattern feeding habits were not changed. However, aggressiveness, sleeping time, and loss of body hair increased in all treated groups as compared to the control group. In the present research, lead acetate administration shows significant on behavioural dysfunction and cytotoxic effect in germinal cells by disturbing sperm production.