Ankylosing spondylosis belongs to a group of Rheumatic disease known as Spondyloarthopathies, which show a strong association with genetic marker HLA- B27. Inflamatory back pain and stiffness are prominent early in the disease where as chronic aggressive disease may produce pain and marked axial Immobility and deformity. In modern medicine there is no promising treatment for the disease. In Ayurveda, it can be co-related to Pristha – graha which can be treated as Vata – Kapha disease. Virechan Karma followed by Basti therapy (Dashamula Niruha and Sahachar taila Anubasan) were administered for that case. Here Basti therapy was applied in Karma Basti schedule and 3 such schedule were performed. After 4 months of completion of the study, all sign and symptoms of Ankylosing spondylosis were diminished. From this result the author had tried the same protocol in other three cases of Ankylosing spondylosis which is also very significant. Further study should be carried out in large sample to establish the rule of said therapy on this particular disease.