Most of the species of Aspergillus are dominant and play vital role in the biodeterioration of fruits. Fungal organisms plays significant role in infection, altering quality and longevity of fruits during the storage. The present investigation is an attempt to screen aflatoxin from. Aspergillus flavus isolates, isolated from different citrus spp. Different isolates of Aspergillus flavus were isolated from Orange (Citrus aurantium), Lemon (Citrus aurantifolia) and Mosambi (Citrus sinensis). These isolates were grown on liquid SMKY medium. By using TLC the cultural filtrate produced by these isolates were screened for Aflatoxin production. About 75% of Aspergillus flavus isolates were found to produce aflatoxin, out which 95% isolates produces aflatoxin (B1), 30% isolates produces B1 and B2, 10% isolates produces B1 and G1 aflatoxin.