The employment in informal sectors has been witnessed of the growing prominence of the labour market in present scenario in India, total contribution of the informal sector to the GDP of the country is more than half. The sector offers the employment opportunity to around 90 % of the total work force. The objective of the present study is to assess prevalence’s of Musculoskeletal Disorders (MSDs) through the assessment of physical health and postural discomfort in several parts of the body workers working in to five different sectors, Chikankari, construction, Sanitary, Brick kilns, Bone craving in Lucknow city Uttar Pradesh. The result of the study reveals that the workers of all five sectors were reported pains at lower back, shoulder, upper arms on the other hand the pains at buttock and thighs were least reported by the workers. The intensity of Musculoskeletal Disorders (MSDs) in tension headache were Maximum (80%, 77%, 47%) among the workers of construction, sanitary, Brick kiln, sectors where as (93%) of ornamental workers and (73%) of Chikankari workers were reported highest problem of irritation in eyes.