Tinospora cordifolia is one of the most important medicinal plants and used in formation of important drug in ayurvedic system of medicine. It is prescribed for many diseases such as fever, general debility, diabetes, urinary infections, jaundice and skin diseases. Aqueous and Methanolic extracts of Tinospora cordifolia was evaluated for their phytochemical analysis. FTIR of methanolic stem, leaf and aqueous stem, leaf is analyzed. Phytochemical screening of Tinospora cordifolia extract revealed the presence of various bioactive components such as Resins, Diterpenes, Flavenoids, Phenols, Carbohydrate, Alkaloids and Amino acids. Further, Fourier Transform Infrared Spectroscopy (FTIR) has strengthened the Phytochemical clarification. In present study phytochemical investigation of Tinospora cordifolia was performed. It showed the presence of Resins, Diterpenes, Flavenoids, Phenols, Carbohydrate, Alkaloids and Amino acids. The presence of bioactive compounds shows the importance of medicinal plants as an efficient of therapeutic agents.