During the process of isolation and identification of leptospira from different parts of Andhra Pradesh Leptonema organisms were also isolated and identified. A total of 458 clinically suspected samples were collected from suspected cases of cattle (26), sheep (42), dogs (13), pigs (15), humans (53), rats (299) and stagnant water from rice field (10) and subjected to isolation of leptospira using EMJH liquid medium. A total of seventeen (17) isolates were recovered from sheep (5), rats (5), pigs (4), humans (2) and rice field water (1) on isolation. Out of 17 isolates two isolates were found to have sluggish motility which is different from that of leptospira and suggestive of leptonema.