A new analytical approach for the determination of triafamone and its metabolites residues in weat plant

A simple and high sensitive method was developed using solid-phase extraction, together with high performance liquid chromatographic method with PDA detection for determination of triafamone and its metabolite residues. The evaluated parameters include the extracts by silica gel SPE cartridge using acetonitrile and 0.1% acetic acid solvents. The method was validated using weat leaves spiked with triafamone and its metabolites at different fortification levels (0.05 and 0.5 µg/g). Average recoveries (using each concentration six replicates) ranged 84-94%, with relative standard deviations less than 2%, calibration solutions concentration in the range 0.02-2.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.02µg/g and 0.05µg/g respectively. Finally the weat leave samples were re analyzed by HPLC.