The development of in vitro propagation of plants holds tremendous potential for the production of high-quality plant-based medicines. Aristalochia indica is used in traditional remedy. In the present study, attempts have been made to develop a simple, reliable and reproducible protocol for micropropagation from different explants of Aristalochia indica. Shoot tip and nodal segments showed elongation without multiplication when either NAA or KIN was used in MS medium. Shoot multiplication was obtained when cytokinins like BAP was used. BAP alone also induced multiple shoots. The regenerated individual shoots were rooted in MS medium containing 1 mg dm-3 indole-3-butyric acid (IBA). Regenerated plants grew well when transferred to soil.