Resident of Baubau City is most using potable water from river and groundwater as drinking water resources. However, rapid developments have deteriorated quality of drinking water sources in Baubau City, Indonesia. For determining water quality has been used Biotic and Polution Index to assess pollution level of Baubau River, Baubau City, Indonesia. Macro invertebrates was collected at 3 station:upper, middle, and lower river from May to September 2014 and at the same time, temperature, total dissolved solid, total suspended solid, pH, dissolved oxygen were analyzed. Pollution level was analyzed used Family Biotic Index (FBI), Belgian Biotic Index (BBI) and STORET-USEPA pollution index methods. The results analysis show that score of FBI were 6.11, 6.53, and 6.67; BBIscore: 4,4, and 3; and water pollution index score on the station 1, 2, and 3 were 2.37, 5.57, and 6.16, respectively. The macroinvertebrates was found, viz., Libelluidae, Parathelphusidae, Athyidae, Palaemonidae, Gerridae, Viviparidae, Thiaridae. Meanwhile Corbiculidae dominated by Gastropodes from Viviparidae and Thiaridae. Conclusion: The overall water quality of the Baubau River from up to lower was moderate to heavy pollution (III-IV).Water bodies are potentially hazardous to public health and that proper sewage treatment and river quality monitoring are needed to warn against hazards to public health.