Atlantia racemosa (Rutaceae) is a small tree or shrub, differing from the other species of Atlantia and it is always unarmed, found in the lower mountain regions of Ceylon, in Southern India and in the western peninsula from the Concan to Travancore. Plant fruit pulp paste works as an antidote to Cobra bite while the leaf juice is taken internally to treat acidity. Leaf decoction is externally applied on itching skin. Seed oil of this plant is used to cure paralysis and chronic rheumatism. Anatomical characters are potentially useful to identify small fragments of commercially important herbal products as well as toxic plants that may be of relevance in forensic investigations. In this investigation, we screen the taxonomical characters execute the identification of Atalantia racemosa with anatomical studies of leaf in order to provide a scientific basis for this important medicinal plant, which these observations on microscopical characters confirmed that A. racemosa has apparent anatomical distinctiveness and peculiarities which differentiate with any adulterant taxa.