Invitro study on antioxidant and antibacterial properties of ethyl acetate extract from the cassia angustifolia

The objective of this research was to probe the in vitro antioxidant and antibacterial activities of ethyl acetate extract from the leaves of Cassia angustifolia. The total phenolic and flavonoid contents were estimated by Folin–Ciocalteu and Aluminium chloride method respectively. Antioxidant activities of C. angustifolia plants were tested on the basis of ABTS (2,2'-azino-bis-3-ethyl benzthiazoline-6-sulphonic acid), radical scavenging assay, inhibition of lipid peroxidation, Super oxide radical scavenging activity, Nitric oxide radical scavenging activity and Metal chelating activity. Similarly, antibacterial activities were performed by disc diffusion method and MIC (Minimum Inhibitory Concentration) against Xanthomonasoryzae pv. oryzae, Pseudomonas fuscovaginaeand Erwiniachrysanthemi. The total phenolic and flavonoid content in ethyl acetate extract of C. angustifoliawas 78.9±1.36 mg GAE/g and 53±2.45μg RE/g respectively. The ethyl acetate extract of C. angustifolia showed significant antioxidant activity. Similarly, the study on antibacterial activity of ethyl acetate extract of C. angustifolia revealed inhibitory activity. However ethyl acetate extract of C. angustifolia showed higher inhibitory zone against Xanthomonas oryzae pv. oryzae, Pseudomonas fuscovaginae and Erwiniachrysanthemi (16, 15.6 and 14.4 mm). This research work has made it clear that ethyl acetate extract of Cassia angustifolia possess excellent antioxidant and antibacterial activity and the extracts can be more widely used in developing countries for the prevention and treatment of ageing and infective related diseases and may be considered as good source for drug discovery.