Background: Preventing and handling infection are one of the indicators of the International Patients Safety Goals which is required to be practiced by a hospital in order to increase service quality. Structured and scheduled supervision is an IPCN’s routineness in which her responsibility, function, and task are intended to increase performance. Management system has to be able to facilitate prevention and handling program which is relevant to the expected result. Objects: To identify the Influence of Organizational Structure and Supervision of Infection Preventive Control Nurse (IPCN) on the Performance of Infection Preventive Control Link Nurse (IPCLN) in Preventing and Handling Infection at the General Hospitals. Methods: The research used quantitative design. The population was 70 IPCNs and IPCLNs, and all of them were used as the samples. The research instrument was NCAT (Nursing Culture Assessment Tools), while the variables of supervision and performance were adopted from Law No. 27/2017 on the Guidance for Preventing and Handling Infection. Bivariate analysis used Spearman Rho. Results: the result showed that there was significant influence of organizational culture on IPCLN’s performance in preventing and handling infection (r=-0.244, p=0.020), IPCN supervised IPCLN’s performance in preventing and handling infection (r=-0.358, p=0.001). Conclusion: Supervision through formative approach should be done IPCLN’s performance would be better if IPCN monitored regularly. Organizational culture in the hospitals was good and reflected comfortable work atmosphere.