Immobilization of glucose oxidase in multi-wall carbon nanotubes on pt modified electrode for high-sensitivity glucose detection

Glucose oxidase sensor is one of the best methods for measuring small amounts of glucose. this sensor can be modified using multi wall carbon nanotubes as complementary substance in the electrode structure with the aim of increasing efficiency.In this paper, Pt electrode, was enriched with carbon nanotubes by an intervening material of amino groups of poly (allylamine) (PAA), and was used as working electrodes (anode) along with a platinum auxiliary electrode and the reference electrode Ag / AgCl (cathode). Working electrode was surrounded by a semi-permeable membrane containing the Phosphate-buffered saline (PBS) with Ph = 4,6,9 enzyme. To evaluate the performance of the constructed biosensor, it was put into a dish containing 0.01, 0.05 and 0.1 M glucose concentrations which were dissolved in doubly distilled water. The produced current and its rate was measured by Potentiostat with transferring the electron from the working electrode to reference electrode which was released by the reaction of glucose in the presence of the enzyme. According to the performed experiments, with increasing concentration, the flow rate of current production is increased and pH deviance from neutral range reduces the flow. Optimal conditions was obtained in concentrations 0.1 M and pH=6, respectively.