Gen bank new Holotype for Coriolopsis byrsina prk-1 with blast rectangle tree view

The Noble prize awarded PCR, an indispensable technique used in biological research for DNA sequencing, DNA-based phylogeny, and functional analysis of genes coupled with ribosomal deoxyribonucleic acid sequencing, polymerase chain reaction and deoxyribonucleic acid sequencing has played a pivotal role in the accurate identification of bacterial/fungal isolates and the discovery of novel bacteria with their 16S rDNA and fungi with their 18S rDNA sequencing in explosive laden soil. Ten different Bacterial isolates and three different Actinomycetes belongs to the genera Acinetobacter, Bacillus, Enterobacter, Enterococcus, Staphylococcus, klebsiella, Aspergillus, Coriolopsis were isolated and identified with their 16S and 18S rDNA sequences and deposited in the The GenBank Maryland USA and MycoBank Utrecht Netherlands (Specimen record 37316). All the isolates were named after the discoverer P Ravikumar, will be preserved in MTCC, India. Sanger dideoxy sequencing technology was employed and the number of base pairs, the base count of A, T, G and C was also studied. To fully utilise 16S/18S rDNA sequencing of bacteria and fungi in explosive laden soils and their bioremediation, the presence of xplA and xplB and other biodegrading gene/s were to be investigated. Coriolopsis byrsina PRK-1 18S ribosomal RNA gene, with the base count 222 a 169 c 246 g 244 t partial sequence with Accession KJ938683, Version KJ938683.1 (GI:675621792 bases 1-881) a novel strain present in the explosive laden soil of cracker industry is discussed here.