Spatholobus purpureus Benth.ex Baker, a medicinally important plant belongs to the family Fabaceae. Traditionally roots are used in the treatment of Haemoresic septicemia in animals. In the present study, the bioactive compounds of Spatholobus purpureus root have been evaluated using GC-MS. The chemical compositions of the acetone extract of S. purpureus were investigated using Perkin-Elmer Gas Chromatography - Mass Spectroscopy. GC-MS analysis of S. purpureus acetone extract revealed the existence of the GC-MS chromatogram of the seventeen peaks presented. The major chemical constituents are like 2-Pentanone, 4-Hydroxy-4-Methyl, Diphenylmethane, Heptacosane, Tetratetracontane, Octacosane (51.44%), , Di-N-Octyl Phthalate, 6H-Benzofuro(3,2-C)(1) Benzopyran,3,9-Dimethoxy, Tritetracontane (16.52%), 6A,12A-Dihydro-6H-(1,3) Dioxolo (5,6) Benzofuro(3,2-C) Chromen-3-Ol, Oxalic acid (6.17%), Decyl 2-Ethyl Hexyl Ester,Pyridine2-Methyl-3-(Trimethylsilyloxy)-4,5-Bis ( (Trimethysilyloxy) Methyl (4.31%), Octadecane,3-Ethyl-5-(2-Ethylbutyl), Di-N-Decylsulfone and Cyclotrisiloxane Hexamethyl (4.47%).