Functional characterization & preparation of e6 & e7 oncogenes of high risk oncogenic humanpapilloma virus-16

In reliable to the fact that changes in amino acid sequence of HPV-16 E6 & E7 proteins might modify the transforming activity of the protein by disturbing the interactions with the EGFR proteins. It was proposed that those variations in the E6 & E7 proteins may affect the transforming potential of HPV-16 owing to change affinity for cellular transcription factors or for viral DNA. In this study, HPV-16 DNA was isolated from cervical cancer tissue using the specific primer designed by the Primer 3 plus software for the HPV antigen E2 gene. The amplified gene was ligated with T vector (pEGTMZ) and transformed into DH5α cells. The plasmid DNA obtained was then established by restriction digestion and sequence analysis which was found to be 99% similar to that obtained in GenBank. Dendrogram was constructed using ClustalW software (online) to get the similarity of the sequence with the existing sequence in the NCBI.