Evaluation of irradiation induced oxidative injury of swiss albino mice using garcinia indica of methanol extract

The current research is to investigate the antioxidant activity of different doses of Garcina Indica against gamma irradiation oxidative damage in the liver tissue with caused single dose of 6Gy. The Malondialdehyde (MDA), Cu,Zn-superoxide dismutase (Cu,Zn-SOD) and selenium-dependent glutathione peroxidase (GSH-Px) are currently considered to be basic markers of oxidative stress. MDA is one of the end-products of the peroxidation of membrane lipids, whereas enzymes Cu,Zn-SOD and GSH-Px belong to the natural antioxidants. The mice were divided into five group with twenty mice each, the group one was with 5mg/kg and group two with 10mg/kg of Garcina Indica extract, and the third group was injected with an isotonic Nacl solution, the fourth group was injected only with Garcina Indica and the fifth was observed as control. Following with a time span of 30 minutes, 6Gy total body irradiation was given to groups one to three in a single dose. The activities were measured in all groups for MDA, SOD and GSH-Px, total body irradiation results in increase in the liver tissue MDA levels and a decrease of SOD and GSH-Px activities and it shows that liver tissue MDA levels in irradiated mice that were pre treated with Garcina Indica were decreased significantly, where SOD and GSH-Px activities were significantly increased. Thus the reported result suggests that Garcina Indica administration prior to irradiation may prevent liver damage by irradiation.