Effect of growth hormones on in-vitro propagation of threatened medicinal species bacopa monnieri L.

An ever increasing demand of uniform medicinal plants based medicines warrants their mass propagation through plant tissue culture strategy. Tissue culture technology is potent and has opened extensive areas of research for biodiversity conservation. Tissue culture protocols have been developed for a wide range of medicinal plants, which includes endangered, rare and threatened plant species, Bacopa monnieri L. is in among these. We studied the effects of various treatment of growth regulators with MS medium on shoot and root induction from auxiliary bud of Bacopa monnieri L., collected from Forest Research Institute and Biotechnology Nursery, Prem Nagar, Dehradun. Growth hormones at different combinations brought out remarkable variations in shoot and root induction, respective length and their survivability. Maximum shoot length, % survivability and minimum days for bud induction were observed in BAP 0.40mg/l, with MS medium, whereas maximum root length % survivability and minimum days taken for root induction were observed in 0.4 mg/l IAA with MS medium.