Chromatography finger print profiling and phytochemical investigation on leaf and bark methanolic extract of ougeinia oojeinensis (roxb.) hochr.

Greenbelts Ougeinia oojeneinensis (Roxb.) Hochr. is a very important medicinal plant in the deciduous forest. Whole parts of the plant are rich in secondary metabolite, which impart miraculous medicinal uses to the plants. Present investigation was designed to evaluate the preliminary phytochemical test on leaf and bark methanolic extract of Ougeniao oojeinensis. The results of the preliminary phytochemical studies confirms the presence of carbohydrate, saponin, starch, flavonoids, steroids, alkaloids, glycosides, terpenoids, and proteins in the methanolic extracts of Ougenia oojeinensis bark and leaves. This study also estimated the Rutin and Qurecetin present in the methanolic leaf and bark extract of the Ougeinia oojeinensis through HPTLC method. The solvent system used for the quantification of quercetin and rutin was Toluene: Ethyl acetate: Formic acid (6: 4: 0.8 v/v/v). The max Rf values of quercetin and rutin were 0.51 and 0.04 respectively. HPTLC fingerprint analysis of leaf and bark extract of Ougeinia oojeinensis can be used as a diagnostic tool for the correct identification of the plant. The present HPTLC profile is useful in differentiating the species from the adulterant and act as a biochemical marker for this medicinally important plant in the pharmaceutical industry and plant systematic studies.