The study was proposed to investigate the changes observed on exercise heart rate of male handball players during a handball match. We selected twelve (12) university represented male handball players from Department of Physical Education and Sports Sciences, Annamalai University, Tamilnadu. However, in the present study goalkeepers were excluded from the study. The players exercise heart rate was measured at every 15th minute during the 60 minute handball match. The results of the study show that peak heart rate of handball players was significantly affected during the handball match as F (3, 33) = 3.071, p < 0.05, ω2 = 0.38. Similarly, mean heart rate of handball players was significantly affected during the handball match as F (3, 33) = 3.300, p < 0.05, ω2 = 0.40. It is concluded that handball players peak heart rate and mean heart rate was affected moderately during the handball match.