Bulk geochemical characteristics include, API gravity, sulfur content, metal contents (V and Ni), bulk composition (saturate, aromatic, polar NSO and asphaltene), normal alkanes and isoprenoids (detected from gas chromatography) were determined for the crude oil samples collected from northern, central and southern province of Gulf of Suez region. These characteristics are used to provide information about source organic matter input, depositional environment and maturation level. The obtained data indicate that these oils were derived from carbonate source rock contain aquatic (algal and bacterial) organic matter with minor terrigenous organic matter contribution that were deposited in marine environments under high reducing conditions and generated at different maturities.