Background: A decline in balance function has been reported in women from 40 years of age onward. Dynamic balance tests are used to predict fall risk in elder women (Plisky et al., 2009; Shaffer et al., 2013; Gribble, 2012; Schmitz, 2007). Objective: 1) To find Normative Y balance test value in Middle aged women and compare Y balance test value in age groups 41-50 and 51-60 years of females. Method: 200 females age between 41-60 years were recruited from community of Charotar region and divided in groups1 & 2. After consideration of inclusion and exclusion criteria informed consent was taken. Details regarding demographic data of all subjects along with Berg Balance Test and Y Balance test was taken. Result: There was no significant difference in Anterior reach distance between Right and left sides in both groups while Posteromedial direction had significant difference in group 2 (P= 0.006). Posterolateral reach distance showed significant difference in between right and left sides in both groups (P=0.02 & P=0.0064). Conclusion: There is significant difference in the reach distance in both the age groups of females with distance comparatively less in the 51-60 year supporting the age related decline in flexibility and balance. Among the 3 directions Posterolateral direction showed significant difference in both the groups.