Introduction: In year 2013, it was assessed that hospitals and medical institutes in Delhi were generating 70 tonnes/day of biomedical waste, of which only 10.7 tonnes/day were being treated properly.Violation of biomedical wastes rules by some hospitals was evident in some of the medical institutes. So, a study was conducted in a tertiary care teaching hospital in Bhopal. The aim of the study was to assess the knowledge and awareness regarding biomedical waste management among health care workers in tertiary care hospital and to identify the areas of concern to take requisite corrective action. Materials and Methods: A cross sectional study was conducted in RKDF institute of medical sciences and research centre Bhopal. Sample size was 100 health care workers. Results: Among 100 questionnaires distributed, 30 respondents comprised of Doctors, 30 Nurses, 40 other (i.e technicians and paramedical staff). Period of work experience varied from less than 5 years (51%), 5–10 years (42%) and more than 10 years (7%) among the Health Care Workers. Conclusion: Knowledge and awareness among health care workers is required for managing hospital waste.