Protein alternatives in diets for warm water and cold water fishes should replace their natural proteins not only in quantity but also in quality to provide adequate levels of essential amino acids (EAA). After making a thorough survey in the district of Midnapore, West Bengal, India, an inventory of non-conventional protein alternatives with special reference to aquatic weeds/macrophytes has been made. On the basis of nutritional value, 12 aquatic macrophytes, has been identified and evaluated to make a data bank of protein alternatives available in West Bengal and also made ranking of the macrophytes on the basis of the dominance in this region.