In the present study antioxidant activities by (2,2-Diphenyl-0 picrylhydrazyl) radical (DPPH), hydrogen peroxide, hydroxyl radical inhibition, hemolysis by hydrogen peroxide assay, reducing power and total antioxidant activities of polyphenolic extract of selected plants leaves were investigated. The total polyphenolic contents of the extract were determined using standard methods. The results of antioxidant activities of poly-phenol extract obtained by different in vitro methods were varied depending on the method used. Nevertheless, polyphenol extract showed significant inhibitory activities in all in vitro reactive oxygen species scavenging, might be attributed due to the high level of polyphenolic compound. Also, these various antioxidant activities were compared to α-tocopherol and L-ascorbic acid as reference antioxidant compounds. These findings provide evidence that the polyphenolic extract of selected plants is a natural source of antioxidant against oxidative damage.
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