Essential oils have many therapeutic properties. In herbal medicine, they are used for their antiseptic properties against infectious fungal diseases, dermatophytes, those of bacterial origin. The aim of our study was to determine the effect of antifungal essential oil of Eucalyptus camaldulensis plant. Eucalyptus is an herb used in traditional therapy. The test adopted is based on the diffusion method in solid medium (sensitivity). This method determines the sensitivity or resistance of the organism vis-à-vis the study sample. The analysis obtained by hydrodistillation of plant samples of an essential oil in a clear yellow color with a return of 0, 99%. Our study reveals that essential oil of Eucalyptus camaldulensis extracted have significant antifungal activity and can successfully replace antibiotic that show their inefficiencies against resistant microorganisms.