Analytical method validation for quantitative estimation of acifluorfen by using rp-hplc

A simple, selective, precise and accurate High Performance Liquid Chromatographic method for the analysis of Acifluorfen in its formulations was developed and validated in the present study. The mobile phase consists of mixture of 0.05% phosphoric acid and acetonitrile in the proportion 20: 80 (v/v). This was found to give sharp peak of Acifluorfen at a run time of 10 min. HPLC analysis of Acifluorfen was carried out at a wave length of 225 nm with a flow rate of 1.0mL/ min. The linear regression analysis data for the calibration curve showed a good linear relationship with a regression coefficient 0.998 in the concentration range of 50% to 150%. The linear regression equation was y =1907x + 270.4. The developed method was employed with a high degree of precision and accuracy for the analysis of Acifluorfen. The method was validated for accuracy, precision, robustness, ruggedness and specificity. The precision, accuracy, sensitivity, short retention time and composition of the mobile phase indicated that this method is useful for the quantification of Acifluorfen.