Α2 –Heremans Schmid Glycoprotein (Ahsg) Gene Thr256ser Polymorphism In Type 2 Diabetes Among South Indians

Alpha 2-Heremans-Schmid glycoprotein (AHSG) is a negative acute-phase reactant and extra osseous calcification inhibitor. Decreased serum concentration of AHSG is independently related to insulin resistance. To identify the single nucleotide polymorphisms (SNPs) of the alpha2-Heremans-Schmid glycoprotein (AHSG) gene and assess their association with Type 2 diabetes in South Indian population. The study included 110 normal healthy volunteers, (68 M: 42 F, mean age 50 ± 7.97 yrs) and 101 T2DM subjects (65 M: 36 F, mean age 52.33 ± 11.12 yrs) of MV hospital for diabetes, Chennai, India. Anthropometric and biochemical parameters were measured. Polymerase Chain Reaction was carried out in the genomic DNAs using primers 5’-GTA AGG CAA CAC TCA GTG A-3’ and 5’-TCA TCA CTG CCA TGT CTA G-3’. PCR-RFLP was performed with the amplicon of 731 bp; using the restriction enzyme SacI that cleaves between T and C (GAGCT*C) and produces 709 and 22 bps bands in agarose gel electrophoresis. Results from our study shows that 98 (89.09 %), 12 (10.91%) and no Controls had, CC (Thr/Thr), CG (Thr/Ser) and G/G (Ser/Ser) polymorphism respectively. 68 (67.32 %), 32 (31.68 %) and 1(0.99%) T2DM patients had CC (Thr/Thr), CG (Thr/Ser) and GG (Ser/Ser) polymorphism respectively. This is statistically significant (p< 0.001). AHSG gene Thr256Ser SNP was significantly associated (p< 0.001) with T2DM among south Indian population.